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BD FACSAria-III Digital Cell Sorter


This powerful instrument, capable of detecting up to 17 parameters and 15 colors simultaneously, makes it possible to identify and isolate even the rarest cell population you are interested in.  Samples can be sorted at single cell-basis with sample agitation and temperature control into two or four tubes (15-mL centrifuge tube to 1.0-mL microtubes), multiwell plates or microscope slides.  It is equipped with Aerosol Management Option (AMO), a device that traps aerosolized particles.  Our AriaIII system is housed in a ClassII Type A2 biosafety cabinet to ensure that samples remain sterile during sorting process.   It is capable of accommodating most cell types with its 70, 85 and 100 micron nozzles.  


Location: Room 462, Apotex Center, University of Manitoba Bannatyne Campus, 750 McDermot Ave., Winnipeg, MB.   


3 Lasers: 1) 20mW 488 nm Solid State blue laser; 2) 15mW 633 nm HeNe red laser; 3) 30 mW 405 nm Solid State violet laser


17 parameters: Forward Scatter, Side Scatter, 6 fluorescent detectors off 488 nm, 3 fluorescent detectors off 633 nm, 6 fluorescent parameters off 405 nm.


Acquisition/Analysis software: FACSDiva





Laser                         Mirror/Filter                Detection Range Fluorochrome:               

blue (488 nm)            502LP, 530/30                515-545 FITC, GFP, Alexa488

      556LP, 585/42                564-606       PE, dsRed, 

      610LP, 616/23                  610-627       PE-Texas Red, PI (live)

      640LP, 670/30                  655-685       PerCP, PE-Cy5, 7-AAD

      685LP, 695/40                   685-715       PE-Cy5.5, PerCP-Cy5.5

      735LP, 780/60                   750-810       PE-Cy7, PE-Alexa750        



red (633 nm)             N/A, 660/20                      650-670                   APC, Alexa647, Cy5 

      690LP, 730/45                   708-752                             Alexa700

      735LP, 780/60                  750-810                              APC-cy7, APC-H7


 

Violet(405 nm)           N/A, 450/40                      430-470                             Pacific blue, Dapi, V450, BV421

      502LP, 510/50                   502-545                              AmCyan, CFP, V500, BV510

      545LP, 560/20                   550-570                              Qdot 565, BrilliantViolet570

      575LP, 585/15                   578-592                              Qdot 585

      595LP, 605/12                   599-611                              Qdot 605, BrilliantViolet605

      630, 655/8BP                    651-663                              Qdot 655, BrilliantViolet650


Notes: 

How to read longpass filter detection range

e.g. 502LP -- wavelenge longer than 502nm will be allowed to pass through


How to read bandpass filter detection range

e.g. 530/30 = 530nm +/- (30/2)nm = 530nm +/- 15nm = 515nm - 545nm