BD FACSCanto-II Digital Flow Cytometry Analyzer 3 Laser System


This powerful instrument, capable of detecting up to 10 parameters and 8 colors simultaneously, is ideal for both clinical and research applications.  Its newly designed loader carousel allows for automatic loading of up to 40 12x75-mm tubes without operator intervention, reducing hands-on time and improving results reliability. 


Location: Room 466, Apotex Center, University of Manitoba Bannatyne Campus, 750 McDermot Ave., Winnipeg, MB.   


3 Lasers: 1) 20mW 488 nm Solid State blue laser; 2) 17mW 633 nm HeNe red laser; 3) 30 mW 405 nm Solid State violet laser


10 parameters: Forward Scatter, Side Scatter, 4 fluorescent detectors off 488 nm, 2 fluorescent detectors off 633 nm, 2 fluorescent parameters off 405 nm.


Acquisition/Analysis software: FACSDiva


FACSCanto_1a.jpgFACSCanto_1b.jpg

Laser                            Mirror/Filter                Detection Range Fluorochrome           

blue (488 nm)                502LP, 530/30           515-545 FITC, GFP, Alexa488

556LP, 585/42               564-606     PE, dsRed,     

655LP, 670LP                 >=670     PerCP, PE-Cy5.5, PI, 7-AAD

735LP, 780/60                750-810     PE-Cy7

 

red (633 nm)                660/20                           650-670 APC, Alexa647, Cy5  

   735LP, 780/60                750-810 APC-cy7, APC-H7

 

Violet (405 nm)             450/50                          425-475 Pacific blue, Dapi, V450, BV421

                                   502LP, 510/50           502-535 AmCyan, V500, BV510


FACSCanto_1b.jpgbanner_2.jpg

Note: 

How to read longpass filter detection range

e.g. 502LP -- wavelenge longer than 502nm will be allowed to pass through


How to read bandpass filter detection range

e.g. 530/30 = 530nm +/- (30/2)nm = 530nm +/- 15nm = 515nm - 545nm