BD LSR-II Digital Flow Cytometry Analyzer


This instrument is one of the most powerful and flexible flow cytometry analyzer on the market.  Its capability of detecting up to 16 parameters and 14 colors simultaneously makes it ideal for multicolor flow cytometry assays that involve a large panel of markers. It can also be used for experiments that cannot be easily performed on other flow cytometry analyzers, such as calcium flux. 

 

Location: Room 536, Basic Medical Science Building, University of Manitoba Bannatyne Campus, 745 Bannatyne Avenue, Winnipeg, MB.   


3 Lasers: 1) 20mW 488 nm Solid State blue laser; 2) 17mW 633 nm HeNe red laser; 3) 30 mW 405 nm Solid State violet laser


16 parameters: Forward Scatter, Side Scatter, 5 fluorescent detectors off 488 nm, 3 fluorescent detectors off 633 nm, 6 fluorescent parameters off 405 nm


Acquisition/Analysis software: FACSDiva



LSR-II_1.jpgLSR-II_1.jpgbanner_2.jpg

Note: 

How to read longpass filter detection range

e.g. 502LP -- wavelenge longer than 502nm will be allowed to pass through


How to read bandpass filter detection range

e.g. 530/30 = 530nm +/- (30/2)nm = 530nm +/- 15nm = 515nm - 545nm

Laser                             Mirror/Filter                 Detection Range  Fluorochrome             

blue (488 nm)               505LP, 530/30             515-545 FITC, GFP, Alexa488

                                   550LP, 575/25             563-587 PE, dsRed, 

                                   600LP, 610/20              600-620 PE-Texas Red, PI (viability)

                                                           635LP, 670/14              656-684 PE-Cy5, PerCP, 7-AAD

                                   735LP, 780/60             750-810 PE-Cy7          

 

red (633 nm)                660/20                        650-670 APC, Alexa647, Cy5

                                   710LP, 730/45             710-752 Alexa700

                                   755LP, 780/60            755-810 APC-cy7, APC-H7

 

Violet (405 nm)             450/40                        430-470 Pacific blue, Dapi, V450, BV421

                                   475LP, 525/50             500-550 AmCyan, CFP, V500, BV510

                                   595LP, 605/12             599-611 BrilliantViolet605

                                   630LP, 655/8             651-659 BrilliantViolet650

                                   690LP, 710/50             685-735 BrilliantViolet711

                                   750LP, 780/60               750-810 BrilliantViolet786